Research / 2022 / Article / Fig 2

Research Article

Downregulation of circ-ZNF609 Promotes Heart Repair by Modulating RNA N6-Methyladenosine-Modified Yap Expression

Figure 2

Knockdown of circ-ZNF609 protects against acute myocardial I/R in vivo. (a) sh-circ-ZNF609 specifically knock down circ-ZNF609 without affecting the expression of linear Zfp609 (human ortholog named as Znf609) mRNA (. ns: nonstatistically significant. wells/group). (b) Expression of circ-ZNF609 in different zones of the heart from mice treated with AAV9-sh-circ-ZNF609 ( mice/group). (c) Attenuated infarct size in heart treated with AAV9-sh-circ-ZNF609 after ischemia/reperfusion injury (I/RI), as evidenced by 2,3,5-triphenyltetrazolium chloride (TTC) staining (; ns: nonstatistically significant. and 6 mice, respectively). (d) Decreased myocardial apoptosis in the mouse I/RI heart treated with AAV9-sh-circ-ZNF609, as evidenced by TUNEL staining ( mice/group, scale μm). I/R: ischemia/reperfusion; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling. Data are presented as (a, c) Independent-sample -test; (b, d) two-way ANOVA test.