Research / 2022 / Article / Fig 4

Research Article

Downregulation of circ-ZNF609 Promotes Heart Repair by Modulating RNA N6-Methyladenosine-Modified Yap Expression

Figure 4

Downregulation of circ-ZNF609 contributes to cardiomyocyte survival in vitro. (a, b) Representative images of immunofluorescence staining and quantification of the relative TUNEL-positive NRCMs showed that circ-ZNF609 regulates NRCM apoptosis ( wells/group, scale μm). (c, d) Western blot analysis of NRCM apoptosis by detection of Bax and Bcl-2 in OGD/R-induced apoptosis model treated with or without circ-ZNF609 overexpression or knockdown ( wells/group). (e, f) Representative images of immunofluorescence staining and quantification of the relative Ki67-positive and pHH3-positive cardiomyocytes treated with or without circ-ZNF609 overexpression or knockdown ( wells/group, scale μm). NC: siRNA negative control; si-circ-ZNF609: siRNA targeted to circ-ZNF609; Plo: circRNA overexpression empty vector Plo-ciR without insert circ-ZNF609 sequence; circ-ZNF609: circ-ZNF609 overexpression construct; NRCM: neonatal rat cardiomyocyte; OGD/R: oxygen-glucose deprivation/reperfusion. Data are presented as (a–d) Two-way ANOVA test; (e, f) independent-sample -test.