Nanozyme-Triggered Cascade Reactions from Cup-Shaped Nanomotors Promote Active Cellular Targeting
Cytotoxicity and antitumor efficacy of GNCs-Pt-ICG/Tf in vitro. (a) HepG2 cell viability incubated with GNCs-Pt-ICG/Tf of various concentrations (0, 1, 5, 10, 25, and 50 μg/mL) for 24 h. Error bars represent the (). (b) The effects of PtNPs and Tf/ICG modification on the cellular uptake efficiency of GNCs-Pt-ICG/Tf. Inset: schematic diagrams of the corresponding nanomaterials. (c) The cellular uptake capability of (i) GNCs-Pt-ICG/Tf, (ii) GNCs-Pt-ICG, (iii) GNCs-ICG/Tf, and (iv) GNCs-ICG for HepG2 cells and (v) GNCs-Pt-ICG/Tf and (vi) GNCs-Pt-ICG for NCTC1469 cells, respectively. Confocal laser scanning microscopy (CLSM) images (d) and corresponding cell mortality (e) of HepG2 cells treated with (I) culture medium, (II) GNCs-Pt-ICG/Tf+laser, (III) GNCs-Pt-ICG/Tf (in dark), (IV) GNCs-Pt-Tf+laser, (V) free ICG+laser, and (VI) GNCs-ICG/Tf+laser, respectively. Scale bar: 50 μm. (f) CLSM images of HepG2 cells cocultured with GNCs-Pt-ICG/Tf at the edge of laser irradiation. Scale bar: 100 μm. ;; Laser: 808 nm, 2 W/cm2.