trans-2-Enoyl-CoA Reductase Tecr-Driven Lipid Metabolism in Endothelial Cells Protects against Transcytosis to Maintain Blood-Brain Barrier Homeostasis
Endothelial Tecr was essential for BRB maturation. (a) Diagram depicting the experimental schedule for EC-specific deletion of Tecr from p3 and analysis at P10, P14, or P18. (b) Confocal images of whole retinal vasculature of the WT and TecriECKO mice after injected 10 kDa dextran at P10 and P18. ECs, IB4 (green); dextran (red). Scale bars, 2 mm. (c, d) Leakage points of WT and TecriECKO retinas at P10 (c) and p18 (d). The leakage points were measured by the number of dextran+ IB4- area. mice per group. , Unpaired -test. (e) Confocal images of retinal vasculature of the WT and TecriECKO mice after injected IgG at P10 and P14. Scale bars, 40 μm. (f, g) Permeability index WT and TecriECKO retinas at P10 (f) and p14 (g). The permeability index is measured by the ratio of IgG+ IB4- area over IgG+ area. mice per group. (f) ; (g) ; unpaired -test. (h) Representative images of Pdgfrβ+ (red, pericyte) coverage onto IB4+ (green) vessels in WT and TecriECKO retinas at P10. Scale bars, 400 μm. (i) Quantitative analysis of pericyte coverage onto vessels in WT and TecriECKO retinas. per group. ns: not significant. Data are expressed as .