trans-2-Enoyl-CoA Reductase Tecr-Driven Lipid Metabolism in Endothelial Cells Protects against Transcytosis to Maintain Blood-Brain Barrier Homeostasis
Loss of Tecr functions in ECs caused BBB breakdown. (a, b) TecriECKO mice displayed BBB leakage. 10 kD dextran (red) and IgG tracer (red) is completely confined within vessels (green, CD31) in WT mice. Tracer-filled parenchyma cells (arrows) surround vessels in the TecriECKO mice brain. Scale bar, 50 μm. (c, d) Permeability index of tracer leakage in brain, as quantified by area of tracer divided by area of vessels per image ( signifying no leakage). mice per group. (c) ; (d) ; unpaired -test. (e) Evans blue examination of BBB at 1 month, 2 months and 3 months. Evans blue examination showed that the BBB integrity was disrupted in TecriECKO mice. mice per group. (f) Quantification of Evans blue staining showed leakage in the brain parenchyma at 1 month, 2 months, and 3 months in TecriECKO mice. mice per group. ; unpaired -test. (g) Representative images of Pdgfrβ+ (red, pericyte) coverage onto IB4+ cerebrovascular vessels in 2 months WT and TecriECKO mice. The brain was coronal sectioned. Every section is 15 μm. Scale bars, 105 μm. (h) Quantitative analysis of pericyte coverage onto cerebrovascular vessels in WT and TecriECKO mice brains. mice per group. ns not significant. Data are expressed as .