Research / 2022 / Article / Fig 5

Research Article

trans-2-Enoyl-CoA Reductase Tecr-Driven Lipid Metabolism in Endothelial Cells Protects against Transcytosis to Maintain Blood-Brain Barrier Homeostasis

Figure 5

Loss of Tecr increased transcellular permeability. (a) TecrKD ECs showed significantly enhanced uptake activity of CTB compared with control. CTB, red; DAPI, blue. Scale bars, 50 μm. (b) Quantitation of CTB uptake per cell. control and TecrKD. , unpaired -test. (c) Western blot analysis and quantification of Cav-1 in control and TecrKD ECs. TecrKD ECs showed a dramatic increase in Cav-1. , unpaired -test. (d) Immunofluorescence images for Plvap of the WT and TecriECKO retinas at P18. Plvap was highly expressed in the TecriECKO retinas. Scale bars, 80 μm. (e) The expression of Plvap is significantly increased in TecriECKO retinas at P18. per group. , unpaired -test. (f) Immunofluorescence images for Plvap of the WT and TecriECKO brains at 2 months. Scale bars, 70 μm. (g) The expression of Plvap is significantly increased in TecriECKO brains at 2 months. per group. , unpaired -test. (h) Lenti-TECR was successfully expressed in Hela cells. (i) Electron microscope images showed the apical plasma membrane of Hela cells after overexpressing human TECR. Overexpressing TECR showed reducing vesicular pit density (arrows) in Hela cells, compared to mock-infected control. Scale bars, 4 μm. Data are expressed as .