Research / 2022 / Article / Fig 2

Research Article

Glymphatic System and Subsidiary Pathways Drive Nanoparticles Away from the Brain

Figure 2

(a) Confocal imaging of AuNCs (green), nuclei (blue), and anti-CD31 antibody-stained microglia (red), white bar represents 50 μm. The orange arrows indicate the co-localization of the endothelia cells and AuNCs. (b) Confocal imaging of AuNCs (green), nuclei (blue), and anti-GFAP antibody-stained astrocytes (red), white bar represents 50 μm. (c) Confocal imaging of AuNCs (green), nuclei (blue), and anti-TMEM-119 antibody-stained endothelia cells (red), white bar represent 100 μm, grey bar represents 30 μm. The white arrows indicate the co-localization of the microglia and AuNCs. (d) The TEM images of AuNCs in brains, the black arrows indicate where the AuNCs lie, while the white arrows show the former exosomes containing AuNCs in multivesicular bodies. En = endothelia cells, As = astrocytes end-feet, Nu = the nuclei of endothelia cells, TJ = tight junction of endothelia cells, Pe = pericytes.