Research / 2022 / Article / Fig 7

Research Article

Glymphatic System and Subsidiary Pathways Drive Nanoparticles Away from the Brain

Figure 7

(a) Illustration of the construction of microglia-deficient modeled mice, the mice needed take the PLX3397 daily for a period of 21 days for experiment, and the AuNCs were i.c. injected 4 h (short interval) before the brains and CLNs were collected for analysis. The FLI of the brains (b) and CLNs (c), the color scale of the brain ranges from 1.0 × 108 to 1.0 × 109, and that for the CLNs ranges from 2.0 × 107 to 5.0 × 107. (d-e) The semi-quantification of brain fluorescent intensity. (f-g) The Au contents in the brain measured by the ICP-MS. (h-i) The semi-quantification of the fluorescent intensity in the CLNs. (j) The interval between i.c. AuNCs treatment and samples collection was prolonged to 12 h. The FLI of the brains (k) and CLNs (l), the color scale of the brain received AuNC@BSA ranges from 1.5 × 107 to 1.5 × 108, while 5.0 × 107 to 5.0 × 108 for that of AuNC@GSH. The color scale for the CLNs ranges from 2.0 × 107 to 5.0 × 107. (m-n) The semi-quantification of brain fluorescent intensity. (o-p) The Au contents in the brain measured by the ICP-MS. (q-r) The semi-quantification of the fluorescent intensity in the CLNs. The unit for fluorescent imaging is (p/sec/cm2/sr)/(μW/cm2).