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Figure 6: Specific labeling of Gram-positive bacteria upon 2 min incubation with activated alkynes. Two kinds of Gram-positive bacteria including Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis) were incubated with three small fluorescent molecules, respectively, including TPA, alkyne-TPA, and the addition product of alkyne-TPA and n-butylamine.