Alt text
Figure 4: mRNA:rRNA pairing exhibits reading frame-specific effects. (a) The left panel depicts schematic of FS reporters with “sticky” or “non-sticky” inserts in the frame 0 between flag and Rluc. The right panels show the relative Rluc activities in transfected HEK293 and MEF cells. Data are shown as means ± SEM, n = 3. (b) The left panel depicts schematic of FS reporters with “sticky” inserts in different frames between flag and Rluc. The right panels show the relative Rluc activities in transfected cells. Data are shown as means ± SEM, n = 3. p <0.01, t-test. (c) Schematic of mRNA:rRNA pairing in the small ribosome subunit when the “sticky” codon is present in frame 1 (top panel) or frame 2 (bottom panel). Inclusion of extra 1 nt in the mRNA loop is more favorable and likely results in –1 FS.