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Figure 5: Mutant 18S rRNA alters global codon “stickiness”. (a) Schematic of mRNA:rRNA pairing in the small ribosome subunit bearing wild type (CAU triplet shown as red) or mutant 18S rRNA (GCC triplet as yellow). Sequence logo shows the probability of individual nucleotides at 5′ end positions of RPFs derived from MEF cells expressing 18S(WT) or 18S(GCC). (b) All 61 sense codons are plotted by the average codon coverage score of RPFs with specified P-site codons (white circle) or 5′ end codons (grey circle) in HEK293 cells expressing wild type (x-axis) or mutant (y-axis) 18S rRNA. (c) All 61 sense codons are plotted by the average IFR of RPFs with specified P-site codons (white circle) or 5′ end codons (grey circle) in HEK293 cells expressing wild type (x-axis) or mutant (y-axis) 18S rRNA. (d) The left panel depicts schematic of FS reporters with a specific sequence insert in frame 0 between flag and Rluc. The right panels show the relative Rluc activities in transfected cells. Data are shown as means ± SEM, n = 3. P <0.01, t-test.