Table 1: Oligos.

Gene namePrimer namePrimer sequence

Non-AUG RenillaRenilla-FCCCAAGCTTCCACCATCGGGATCCCCACCATCGGAATTCacttcgaaagtttatgatc
Renilla-RCCCTCGAGcccctagaattattgttc

Flag frame 0Flag-0-SAGCTTGCCACCATGgattacaaggacgacgacgataagG
Flag-0-ASGATCCcttatcgtcgtcgtccttgtaatcCATGGTGGCA

+1 FSFS1-SGATCCACGATCAAGCTGTTGGTGCTGCG
FS1-ASAATTCGCAGCACCAACAGCTTGATCGTG

−1 FSFS2-SGATCCACGATCAAGCTGTTGGTGCTGCAG
FS2-ASAATTCTGCAGCACCAACAGCTTGATCGTG

Non-sticky frame 0NS-0-SGATCCGGAGACGGCGGAGGTGGAGACG
NS-0-ASAATTCGTCTCCACCTCCGCCGTCTCCG

Non-sticky FS1NS-FS1-SGATCCGGAGACGGCGGAGGTGGAGACCG
NS-FS1-ASAATTCGGTCTCCACCTCCGCCGTCTCCG

Non-sticky FS2NS-FS2-SGATCCGGAGACGGCGGAGGTGGAGACCAG
NS-FS2-ASAATTCTGGTCTCCACCTCCGCCGTCTCCG

+1 FS frame 1+1 FS-SGATCCCACGATCAAGCTGTTGGTGCTGG
+1 FS-ASAATTCCAGCACCAACAGCTTGATCGTGG

−1 FS frame 2+1 FS-SGATCCCAACGATCAAGCTGTTGGTGCTGG
+1 FS-ASAATTCCAGCACCAACAGCTTGATCGTTGG