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Figure 5: Biochemical assays identifying the lesion-containing strand. (a) Schematic illustrating the UvrB translocation assay. (b) The reactions analyzed by native PAGE. (c) 50-mer DNA substrates used for UvrB crosslinking assay. A single fluorescein-adducted thymine (Flu-dT) is located at various positions either on the same (Set I) or opposite (Set II) strand to a disulfide-bearing tether (XL). (d) Crosslinked products analyzed by SDS-PAGE. Control reactions contain the unmodified duplex DNA (U/D) and duplex DNA containing either fluorescein (Flu) or disulfide-bearing tether (XL) alone. (e) The average and standard deviation of three independent experiments is shown for each DNA substrate shown in Figure 6(c).